Part:BBa_K3143002
Phd antitoxin
Antitoxin component of a type II toxin-antitoxin (TA) system. Phd is a labile antitoxin that binds to cognate doc toxin and neutralizes its ability to phosphorylate host EF-Tu. The phd/doc family is one the smallest families of toxin–antitoxin modules and was first discovered as a plasmid addiction module on E.coli bacteriophage P1. phd and doc proteins function in unison to stabilize plasmid number by inducing a lethal response to P1 plasmid prophage loss.[1] See Doc toxin BBa_K3143001.
Characterization
We tested the effects of Phd-Doc. We used different inducible promoters to control the expression of Phd and Doc proteins, respectively. As can be seen from Fig 1, bacterial growth was completely inhibited after induction of Doc protein expression. When both Phd and Doc proteins are expressed, the growth of bacteria is no longer affected by Doc toxic proteins.
Figure 1: A The process for bacteria growth imaging B The imaging of bacteria growth of different groups after 24 hours
Reference
1. Lehnherr H, Maguin E, Jafri S, et al. Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained[J]. Journal of molecular biology, 1993, 233(3): 414-428.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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